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NIH grant 1K99GM166692-01: Development of Activity-Based Probes to Decipher Targets and Distributions of Reactive Metabolites
Institute: {'code': 'GM', 'abbreviation': 'NIGMS', 'name': 'National Institute of General Medical Sciences', 'admin_org_id': '12500024', 'admin_funding_url': ''}. PI: Alexander K Hurben. Award: $125000.
Start: 2026-08-06T00:00:00.
Project Summary/Abstract
Reactive oxygen species (ROS) and aldehydes are ubiquitous small molecules that play
a diverse array of important roles in human health and disease. Various ROS and
aldehyde metabolites are classified as carcinogens, and aldehyde exposure is also
connected to a variety of diseases ranging from chronic liver disorders to cancer, diabetes,
and cardiovascular and neurodegenerative diseases. Simultaneously, the body produces
these reactive metabolites during normal physiology, primarily through various metabolic
processes. We seek to develop and apply new chemical reagents for selective proteomics
and multiplexed detection of ROS and aldehydes in living systems to identify their
molecular sources and targets, with the long-term goal of understanding how and in what
context these reactive metabolites contribute to both health and disease. This application
will focus on the development and application of new chemoproteomic methods to identify
targets of ROS aldehydes across the proteome and new activity-based probes enabling
multiplexed quantification of numerous aldehyde species in genetic models where
aldehyde metabolism is compromised. Specific aims include applying unbiased activity-
based protein profiling (ABPP) methods to tryptophan-, lysine, and arginine-derived
targets of ROS and aldehydes in whole proteomes, and developing and applying activity-
based probes to enable multiplexed detection and quantification biological aldehyde flux.document fetched 2026-08-16 06:34